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Anti survivin Mouse Monoclonal Antibody

Price$239.00
BrandNewEastBio   
Place of OriginUSA
Catalog Number26131
ImmunogenMouse
Size100 μl
Technical InformationView technical data
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  • Summary

    Survivin [26131]
    [100 µL]

    Catalog Number:  26131
    Gene Symbol:  BIRC5; API4; EPR-1
    Description:   anti survivin Mouse Monoclonal Antibody
    Background:   Survivin is a member of the inhibitor of apoptosis (IAP) family. The survivin protein negatively regulate of programmed cell death by inhibiting caspase. Survivin protein is high expressed in most human tumours and fetal tissue, but is absent in differentiated cells. This makes survivin an ideal target for cancer therapy. Survivin is only expressed in the G2-M phase cells. It also plays a role in regulating mitosis.
    Immunogen:   Purified survivin protein, human origin.
    Applications:   ELISA, WB, IHC
    Recommended Dilutions:   
    ELISA: 1:1000-1:5000 
    WB: 1:100-1:1000 
    IHC: 1:50-1:200
    Concentration:   1mg/ml
    Host:   Mouse
    Format:   Liquid
    Clonality:   Monoclonal
    Isotype:   
    Purity:   Purified from ascites
    Storage buffer:
    Preservative:  no
    Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Recognizes survivin of vertebrates.
    Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
    Download Datasheet:  
    Western blot:  
    Western blot analysis of recombinant PTEN protein. Purified His-tagged Survivin protein was blotted with anti survivin mouse monoclonal antibody (Cat. #26131).
    Immunohistochemistry:   
    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with anti survivin monoclonal antibody (Cat. #26131). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:50 dilution) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (1:50 dilution) was used as secondary antibody.



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