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Antibodies for Mutated Oncogene

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Anti FBXW7(R479L) Mouse Monoclonal Antibody

Price$495.00
BrandNewEastBio   
Place of OriginUSA
Catalog Number26347
ImmunogenMouse
Size100 μl
Technical InformationView technical data
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  • Summary

    FBXW7(R479L) [26347]
    [100ul]

    Catalog Number:  26347
    Gene Symbol:  FBW6; FBW7; FBXW7; FBX30; CDC4; SEL10
    Description:   Anti FBXW7(R479L) Mouse Monoclonal Antibody
    Background:   F-box/WD repeat-containing protein 7 is a protein that in humans is encoded by the FBXW7 gene. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. Mutations in this gene are detected in ovarian, breast and colorectal cancer cell lines, implicating the gene's potential role in the pathogenesis of human cancers.
    Immunogen:   A synthetic peptide from the internal region of FBXW7 which includes the mutation of R479L, human origin.
    Applications:   ELISA, WB
    Recommended Dilutions:   
    ELISA: 1:1000-1:5000 
    WB: 1:500-1:1000
    Concentration:   1 mg/ml
    Host:   Mouse
    Format:   Liquid
    Clonality:   Monoclonal
    Isotype:   
    Purity:   Purified from ascites
    Storage buffer:
    Preservative:  no
    Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Recognizes R479L mutant, but not wild-type FBXW7 of vertebrates.
    Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
    Download Datasheet:  
    Western blot:  
    Western blot analysis of recombinant FBXW7(R479L) and wildtype proteins. Purified His-tagged FBXW7(R479L) protein (lane 2) and corresponding wildtype protein (lane 1) were blotted with Anti FBXW7(R479L) monoclonal antibody (Cat. #26347).
    Immunofluorescence:  
    Immunofluorescence of cells expressing FBXW7 proteins with Anti FBXW7(R479L) antibody. HEK293T cells were transfected with pCDNA3-GFP-FBXW7 (WT) plasmid (left column) or pCDNA3-GFP-FBXW7(R479L) plasmid (right column), then fixed and stained with Anti FBXW7(R479L) monoclonal antibody (Cat. #26347).



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